FineTest
SKU:EM1990
Mouse BCAT1(Branched-chain-amino-acid aminotransferase, cytosolic) ELISA Kit
Mouse BCAT1(Branched-chain-amino-acid aminotransferase, cytosolic) ELISA Kit
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Product Name
Mouse BCAT1(Branched-chain-amino-acid aminotransferase, cytosolic) ELISA Kit
Alias
Branched-chain-amino-acid aminotransferase, cytosolic ELISA Kit, BCAT（c） ELISA Kit, Protein ECA39 ELISA Kit, Eca39 ELISA Kit, Bcat1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
BCAT1 ELISA Kit allows for the in vitro quantitative determination of BCAT1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of BCAT1 and the recovery rates were calculated by comparing the measured value to the expected amount of BCAT1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-94 |
90 |
|
EDTA plasma(n=5) |
90-102 |
94 |
|
heparin plasma(n=5) |
90-104 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BCAT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-97% |
81-105% |
83-103% |
|
EDTA plasma(n=5) |
93-101% |
93-101% |
95-100% |
|
heparin plasma(n=5) |
90-96% |
90-97% |
90-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
