FineTest
SKU:ECA0098
Canine IL-5(Interleukin-5) ELISA Kit
Canine IL-5(Interleukin-5) ELISA Kit
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Product Name
Canine IL-5(Interleukin-5) Elisa kit
Alias
Interleukin-5 ELISA Kit, IL-5 ELISA Kit, Eosinophil differentiation factor ELISA Kit, T-cell replacing factor ELISA Kit, TRF ELISA Kit, IL5 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-5 Elisa kit allows for the in vitro quantitative determination of IL-5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Canine
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-5 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-5 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
82-98 |
91 |
|
EDTA plasma(n=5) |
81-105 |
95 |
|
heparin plasma(n=5) |
80-104 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
83-101% |
85-104% |
82-93% |
|
EDTA plasma(n=5) |
91-101% |
90-99% |
90-94% |
|
heparin plasma(n=5) |
90-99% |
90-98% |
90-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
