FineTest
SKU:EM1998
Mouse ova sigg2b(Ovalbumin Specific IgG2b) ELISA Kit
Mouse ova sigg2b(Ovalbumin Specific IgG2b) ELISA Kit
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Product Name
Mouse OVA sIgG2b (Ovalbumin Specific IgG2b) ELISA Kit
Alias
OVA sIgG2b ELISA Kit, Ovalbumin Specific IgG2b ELISA Kit
Detection Method
Capture ELISA
Application
OVA sIgG2b ELISA Kit allows for the in vitro quantitative determination of OVA sIgG2b concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Mouse
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of OVA sIgG2b and the recovery rates were calculated by comparing the measured value to the expected amount of OVA sIgG2b in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
82-104 |
96 |
|
EDTA plasma(n=5) |
83-105 |
96 |
|
heparin plasma(n=5) |
86-98 |
91 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OVA sIgG2b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
80-105% |
83-99% |
85-105% |
|
EDTA plasma(n=5) |
90-100% |
90-100% |
90-100% |
|
heparin plasma(n=5) |
91-97% |
91-95% |
94-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
