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Rat Htr1a(5-hydroxytryptamine receptor 1A)ELISA Kit
Rat Htr1a(5-hydroxytryptamine receptor 1A)ELISA Kit
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Product Name
Rat Htr1a(5-hydroxytryptamine receptor 1A)ELISA Kit
Alias
5-hydroxytryptamine receptor 1A ELISA Kit, 5-HT-1A ELISA Kit, 5-HT1A ELISA Kit, Serotonin receptor 1A ELISA Kit, 5ht1a ELISA Kit, Htr1a ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Htr1aELISA Kit allows for the in vitro quantitative determination of Htr1a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Rat
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Htr1a and the recovery rates were calculated by comparing the measured value to the expected amount of Htr1a in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
82-101 |
93 |
|
EDTA plasma(n=5) |
88-97 |
93 |
|
heparin plasma(n=5) |
84-103 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Htr1a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-100% |
80-102% |
83-105% |
|
EDTA plasma(n=5) |
90-98% |
94-101% |
93-98% |
|
heparin plasma(n=5) |
94-98% |
92-99% |
92-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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