FineTest
SKU:EH9136
Human HLA-DRA(HLA class II histocompatibility antigen, DR alpha chain) ELISA Kit
Human HLA-DRA(HLA class II histocompatibility antigen, DR alpha chain) ELISA Kit
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Product Name
Human HLA-DRA(HLA class II histocompatibility antigen, DR alpha chain) ELISA Kit
Alias
HLA class II histocompatibility antigen, DR alpha chain ELISA Kit, MHC class II antigen DRA ELISA Kit, HLA-DRA1 ELISA Kit, HLA-DRA ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
HLA-DRA ELISA Kit allows for the in vitro quantitative determination of HLA-DRA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of HLA-DRA and the recovery rates were calculated by comparing the measured value to the expected amount of HLA-DRA in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
10-73 |
40 |
|
EDTA plasma(n=5) |
5-97 |
50 |
|
heparin plasma(n=5) |
20-87 |
40 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HLA-DRA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
26-78% |
15-85% |
0-97% |
|
EDTA plasma(n=5) |
5-96% |
38-92% |
5-78% |
|
heparin plasma(n=5) |
38-75% |
21-98% |
4-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
