FineTest
SKU:EP0210
Porcine GHRL(Ghrelin) ELISA Kit
Porcine GHRL(Ghrelin) ELISA Kit
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Product Name
Porcine GHRL(Ghrelin) ELISA Kit
Alias
Ghrelin ELISA Kit, GHRL ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
GHRL ELISA Kit allows for the in vitro quantitative determination of GHRL concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.12-200pg/ml
Sensitivity
< 1.875pg/ml
Species
Porcine
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of GHRL and the recovery rates were calculated by comparing the measured value to the expected amount of GHRL in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
95-99 |
98 |
|
EDTA plasma(n=5) |
96-101 |
98 |
|
heparin plasma(n=5) |
96-105 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GHRL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-105% |
86-98% |
98-105% |
|
EDTA plasma(n=5) |
90-101% |
91-97% |
95-100% |
|
heparin plasma(n=5) |
93-100% |
91-100% |
90-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
