FineTest
SKU:EH4073
Human ITCH(E3 ubiquitin-protein ligase Itchy homolog) ELISA Kit
Human ITCH(E3 ubiquitin-protein ligase Itchy homolog) ELISA Kit
Couldn't load pickup availability
Product Name
Human ITCH(E3 ubiquitin-protein ligase Itchy homolog) Elisa Kit
Alias
E3 ubiquitin-protein ligase Itchy homolog ELISA Kit, Itch ELISA Kit, Atrophin-1-interacting protein 4 ELISA Kit, AIP4 ELISA Kit, HECT-type E3 ubiquitin transferase Itchy homolog ELISA Kit, NFE2-associated polypeptide 1 ELISA Kit, NAPP1 ELISA Kit, ITCH ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
ITCH Elisa Kit allows for the in vitro quantitative determination of ITCH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of ITCH and the recovery rates were calculated by comparing the measured value to the expected amount of ITCH in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
96-103 |
99 |
|
EDTA plasma(n=5) |
96-104 |
99 |
|
heparin plasma(n=5) |
95-103 |
100 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ITCH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-105% |
91-105% |
91-105% |
|
EDTA plasma(n=5) |
89-100% |
89-100% |
90-98% |
|
heparin plasma(n=5) |
90-99% |
94-99% |
91-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
