FineTest
SKU:EM2057
Mouse ERK1/2(Extracellular Signal Regulated Kinase 1/2)ELISA Kit
Mouse ERK1/2(Extracellular Signal Regulated Kinase 1/2)ELISA Kit
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Product Name
Mouse ERK1/2(Extracellular Signal Regulated Kinase 1/2)ELISA Kit
Alias
ERK1/2 ELISA Kit, Extracellular Signal Regulated Kinase 1/2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
ERK1/2ELISA Kit allows for the in vitro quantitative determination of ERK1/2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.781-50ng/ml
Sensitivity
< 0.469ng/ml
Species
Mouse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of ERK1/2 and the recovery rates were calculated by comparing the measured value to the expected amount of ERK1/2 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-98 |
90 |
|
EDTA plasma(n=5) |
93-103 |
99 |
|
heparin plasma(n=5) |
86-99 |
91 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ERK1/2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-100% |
88-104% |
85-97% |
|
EDTA plasma(n=5) |
84-101% |
84-90% |
85-92% |
|
heparin plasma(n=5) |
83-97% |
80-95% |
83-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
