FineTest
SKU:ER2032
Rat IDO1(Indoleamine 2,3-dioxygenase 1)ELISA Kit
Rat IDO1(Indoleamine 2,3-dioxygenase 1)ELISA Kit
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Product Name
Rat IDO1(Indoleamine 2,3-dioxygenase 1)ELISA Kit
Alias
IDO1 ELISA Kit, IDO-1 ELISA Kit, Indoleamine 2,3-dioxygenase 1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IDO1ELISA Kit allows for the in vitro quantitative determination of IDO1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IDO1 and the recovery rates were calculated by comparing the measured value to the expected amount of IDO1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-98 |
90 |
|
EDTA plasma(n=5) |
91-100 |
96 |
|
heparin plasma(n=5) |
88-105 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IDO1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-103% |
91-103% |
97-102% |
|
EDTA plasma(n=5) |
83-97% |
86-99% |
83-101% |
|
heparin plasma(n=5) |
85-99% |
83-100% |
82-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
