Phospho-Akt (S473) mouse Monoclonal Antibody(7F9)

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

The PHOSPHO-AKT (S473) Mouse Monoclonal Antibody (7F9) is a highly specific and reliable tool designed for the detection and analysis of phosphorylated AKT at serine 473 residue. This antibody is produced using advanced monoclonal antibody technology, ensuring exceptional quality and performance.

The PHOSPHO-AKT (S473) Mouse Monoclonal Antibody (7F9) exhibits a high affinity towards phosphorylated AKT, making it an ideal choice for various applications, including western blotting, immunoprecipitation, and immunofluorescence. Its exceptional specificity allows for accurate and precise detection of phosphorylated AKT, enabling researchers to gain valuable insights into the activation and regulation of this critical signaling molecule.

This antibody is meticulously validated to ensure consistent and reproducible results. It has been extensively tested in various experimental conditions and has shown excellent performance in detecting phosphorylated AKT in a wide range of sample types, including cell lysates and tissue extracts.

The PHOSPHO-AKT (S473) Mouse Monoclonal Antibody (7F9) is supplied in a convenient and ready-to-use format, eliminating the need for time-consuming and complex antibody preparation. Its high sensitivity and low background ensure reliable and accurate results, even with low abundance samples.

With its exceptional quality, specificity, and reliability, the PHOSPHO-AKT (S473) Mouse Monoclonal Antibody (7F9) is an indispensable tool for researchers studying AKT signaling pathways and its involvement in various cellular processes.