WT1 Polyclonal Antibody

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.

The WT1 polyclonal antibody is a highly specific and sensitive immunological reagent designed for the detection and quantification of the Wilms tumor protein 1 (WT1) in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human WT1 protein, followed by affinity purification using a WT1-specific antigen. The resulting polyclonal antibody exhibits excellent performance in a range of applications, including Western blotting, immunohistochemistry, and ELISA. It recognizes both the full-length and truncated isoforms of WT1 protein, and shows minimal cross-reactivity with other related proteins. The WT1 polyclonal antibody is supplied as a liquid formulation in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative. It is available in various sizes and concentrations to meet the needs of different research projects. With its high quality and reliability, the WT1 polyclonal antibody is an essential tool for studying the role of WT1 protein in cancer biology, developmental biology, and stem cell research.