TNNT1 Polyclonal Antibody

This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene.

The TNNT1 polyclonal antibody is a highly specific and sensitive immunological reagent designed for the detection and quantification of troponin T type 1 (TNNT1) protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human TNNT1 protein. The resulting polyclonal antibody exhibits high affinity and specificity towards TNNT1 protein, making it an ideal tool for various applications in research and diagnostics.

The TNNT1 polyclonal antibody is suitable for use in various immunoassays, including Western blotting, ELISA, immunohistochemistry, and immunofluorescence. It has been validated for use in human, mouse, and rat samples, and has been shown to detect TNNT1 protein in a variety of tissues, including heart, skeletal muscle, and smooth muscle. This antibody is also suitable for use in studies of cardiac injury and disease, as TNNT1 is a well-established biomarker for myocardial infarction.

The TNNT1 polyclonal antibody is supplied as a purified IgG fraction, and is available in various sizes to meet the needs of different applications. It is recommended for use at a dilution of 1:1000 for Western blotting, and 1:200 for immunohistochemistry and immunofluorescence. This antibody is produced under strict quality control standards, ensuring consistent performance and reproducibility.

In summary, the TNNT1 polyclonal antibody is a highly specific and sensitive reagent that is ideal for the detection and quantification of TNNT1 protein in various biological samples. Its versatility and reliability make it an essential tool for researchers and clinicians working in the fields of cardiovascular disease, muscle biology, and biomarker discovery.