SPHK1 Polyclonal Antibody

The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

The SPHK1 Polyclonal Antibody is a highly specific and sensitive tool for the detection and quantification of sphingosine kinase 1 (SPHK1) protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human SPHK1 protein. The resulting polyclonal antibody exhibits high affinity and specificity towards SPHK1 protein, making it an ideal reagent for various applications such as Western blotting, immunohistochemistry, and ELISA.

The SPHK1 Polyclonal Antibody is validated for use in human, mouse, and rat samples, and has been extensively tested for its specificity and sensitivity. It has been shown to detect endogenous SPHK1 protein in various cell lines and tissues, and can be used to monitor changes in SPHK1 expression levels under different experimental conditions.

This antibody is supplied as a purified IgG fraction, and is available in various sizes to suit different experimental needs. It is recommended to use this antibody at a dilution of 1:1000 for Western blotting, and 1:200 for immunohistochemistry. The SPHK1 Polyclonal Antibody is a reliable and high-quality reagent that can help researchers to better understand the role of SPHK1 in various biological processes.