SACS Polyclonal Antibody

This gene encodes the sacsin protein, which includes a UbL domain at the N-terminus, a DnaJ domain, and a HEPN domain at the C-terminus. The gene is highly expressed in the central nervous system, also found in skin, skeletal muscles and at low levels in the pancreas. This gene includes a very large exon spanning more than 12.8 kb. Mutations in this gene result in autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS), a neurodegenerative disorder characterized by early-onset cerebellar ataxia with spasticity and peripheral neuropathy. The authors of a publication on the effects of siRNA-mediated sacsin knockdown concluded that sacsin protects against mutant ataxin-1 and suggest that "the large multi-domain sacsin protein is able to recruit Hsp70 chaperone action and has the potential to regulate the effects of other ataxia proteins" (Parfitt et al., PubMed: 19208651).

The SACS Polyclonal Antibody is a highly specific and sensitive immunological reagent designed for the detection and quantification of the SACS protein. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to a unique sequence of the SACS protein. The resulting polyclonal antibody exhibits high affinity and specificity towards the SACS protein, making it an ideal tool for various applications in research and diagnostics.

The SACS Polyclonal Antibody is suitable for use in a variety of immunoassays, including Western blotting, immunohistochemistry, and ELISA. It has been validated for use in human, mouse, and rat samples, and has been shown to detect endogenous levels of the SACS protein in various tissues and cell lines. The antibody is supplied as a purified IgG fraction, and has been tested for purity and specificity by SDS-PAGE and ELISA.

The SACS Polyclonal Antibody is a valuable tool for researchers studying the role of the SACS protein in various biological processes, including neurodegenerative diseases, ataxia, and cerebellar development. It can also be used in clinical settings for the diagnosis and monitoring of SACS-related disorders. With its high specificity and sensitivity, the SACS Polyclonal Antibody is a reliable and effective reagent for the detection and quantification of the SACS protein.