MLK1/2(Phospho Thr312/266) Polyclonal Antibody

Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.,Activates the JUN N-terminal pathway.,PTM:Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site.,Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,Contains 1 protein kinase domain.,Contains 1 SH3 domain.,subunit:Homodimer.,tissue specificity:Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.,

The MLK1/2(Phospho Thr312/266) Polyclonal Antibody is a highly specific and sensitive tool designed for the detection and quantification of MLK1/2 protein phosphorylated at Thr312/266 residues. This antibody is produced using advanced techniques and rigorous quality control measures to ensure optimal performance and reliability.

The MLK1/2 protein, also known as mixed lineage kinase 1/2, plays a crucial role in various cellular processes, including cell growth, differentiation, and apoptosis. Phosphorylation of MLK1/2 at Thr312/266 residues is a key regulatory event that modulates its activity and downstream signaling pathways.

This polyclonal antibody is raised in rabbits using a synthetic peptide corresponding to the phosphorylated Thr312/266 region of MLK1/2 protein. It exhibits high affinity and specificity towards the phosphorylated form of MLK1/2, enabling accurate detection and quantification in various experimental settings.

The MLK1/2(Phospho Thr312/266) Polyclonal Antibody has been extensively validated for its performance in Western blotting, immunohistochemistry, and immunofluorescence applications. It demonstrates excellent sensitivity and minimal cross-reactivity with non-phosphorylated MLK1/2 protein, ensuring reliable and specific results.

This antibody is supplied as a liquid formulation in a convenient size of 100 µg, allowing for multiple experiments. It is recommended to store the antibody at -20°C for long-term stability. Prior to use, it is advised to aliquot the antibody to avoid repeated freeze-thaw cycles.

In summary, the MLK1/2(Phospho Thr312/266) Polyclonal Antibody is a valuable tool for researchers studying the phosphorylation status and functional implications of MLK1/2 protein. Its high specificity, sensitivity, and reliable performance make it an essential component of any study involving MLK1/2 signaling pathways.