Histone H3(Phospho Thr45) Polyclonal Antibody

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

The Histone H3(Phospho Thr45) Polyclonal Antibody is a highly specific and sensitive tool for the detection and analysis of phosphorylated Histone H3 at Thr45. This antibody has been meticulously developed and rigorously validated to ensure optimal performance and reliability in various applications, including Western blotting, immunohistochemistry, and immunofluorescence.

Histone H3 is a crucial component of chromatin structure and plays a pivotal role in gene regulation and DNA packaging. Phosphorylation of Histone H3 at Thr45 has been implicated in various biological processes, such as cell cycle progression, DNA repair, and transcriptional activation. Therefore, the ability to accurately detect and quantify this phosphorylation event is of utmost importance in understanding the underlying mechanisms of these processes.

The Histone H3(Phospho Thr45) Polyclonal Antibody exhibits exceptional specificity towards the phosphorylated form of Histone H3 at Thr45, ensuring minimal cross-reactivity with non-phosphorylated forms or other histone variants. This specificity is further enhanced by the use of high-quality antigen affinity purification, which eliminates any potential non-specific binding.

In addition to its specificity, this antibody also demonstrates excellent sensitivity, allowing for the detection of low levels of phosphorylated Histone H3 at Thr45. This attribute is particularly advantageous when working with limited sample quantities or when studying subtle changes in phosphorylation levels.

Furthermore, the Histone H3(Phospho Thr45) Polyclonal Antibody has been extensively validated in various experimental conditions and sample types, ensuring consistent and reliable results. Its compatibility with multiple applications, including Western blotting, immunohistochemistry, and immunofluorescence, provides researchers with the flexibility to choose the most suitable technique for their specific experimental needs.

In summary, the Histone H3(Phospho Thr45) Polyclonal Antibody is a highly specific, sensitive, and reliable tool for the detection and analysis of phosphorylated Histone H3 at Thr45. Its exceptional performance and versatility make it an invaluable asset for researchers studying chromatin structure, gene regulation, and various biological processes.