EIF3A Polyclonal Antibody

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.,mass spectrometry: PubMed:17322308,PTM:Phosphorylated. Phosphorylation is enhanced upon serum stimulation.,Belongs to the eIF-3 subunit A family.,Contains 1 PCI domain.,subunit:Interacts with EIF4G1 (By similarity). Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3L and EIF3K. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of FRAP1 and RAPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with KRT7 and PIWIL2.,

The EIF3A Polyclonal Antibody is a highly specific and reliable tool for the detection and analysis of EIF3A protein expression in various biological samples. This antibody has been extensively validated for its superior performance in Western blotting, immunohistochemistry, and immunofluorescence applications.

The EIF3A Polyclonal Antibody is produced using high-quality materials and advanced techniques to ensure optimal sensitivity and specificity. It has been rigorously tested to ensure minimal cross-reactivity with other proteins, guaranteeing accurate and reliable results.

This antibody exhibits excellent performance in a wide range of species, including human, mouse, and rat, making it suitable for diverse research applications. It is designed to recognize the endogenous levels of EIF3A protein, enabling precise quantification and analysis of its expression in various tissues and cell types.

The EIF3A Polyclonal Antibody is supplied as a liquid formulation, allowing for easy and convenient handling. It is provided in a ready-to-use format, eliminating the need for time-consuming and complicated preparation steps. The antibody is also available in different sizes to accommodate various experimental needs.

With its exceptional specificity, sensitivity, and versatility, the EIF3A Polyclonal Antibody is an indispensable tool for researchers studying EIF3A protein function and its involvement in cellular processes. Its reliable performance and ease of use make it an ideal choice for both basic research and clinical applications.