DFFB Polyclonal Antibody

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined.

The DFFB polyclonal antibody is a highly specific and sensitive immunological reagent designed for the detection and quantification of DFFB protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human DFFB protein. The resulting polyclonal antibody exhibits high affinity and specificity towards DFFB protein, making it an ideal tool for various applications such as Western blotting, immunohistochemistry, and ELISA.

The DFFB polyclonal antibody is purified using a combination of protein A and protein G chromatography, ensuring high purity and minimal cross-reactivity with other proteins. This antibody is supplied as a liquid formulation in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative. The DFFB polyclonal antibody is suitable for use in a wide range of research applications, including cancer research, apoptosis studies, and drug discovery.

In summary, the DFFB polyclonal antibody is a reliable and high-quality immunological reagent that offers exceptional sensitivity and specificity for the detection and quantification of DFFB protein. Its superior performance and versatility make it an essential tool for researchers in various fields of life sciences.