CUL-1 Polyclonal Antibody

Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(SKP2) directs ubiquination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquination of ORC1L, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1 (By similarity). SCF(FBXO11) does not seem to direct ubiquitination of TP53. Interacts with FBXW8. Interacts with CUL7; the interaction seems to be mediated by FBXW8.,pathway:Protein modification; protein ubiquitination.,PTM:Neddylated; which enhances the ubiquitination activity of SCF. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.,Belongs to the cullin family.,subunit:Component of multiple SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complexes formed of CUL1, SKP1A, RBX1 and a variable F-box domain-containing protein as substrate-specific subunit. Component of the SCF(BTRC) complex containing BTRC. Component of the SCF(FBXW11) complex containing FBXW11. Component of the SCF(SKP2) complex containing SKP2, in which it interacts directly with SKP1, SKP2 and RBX1. Component of the SCF(FBXW2) complex containing FBXw2. Component of the SCF(FBXO32) complex containing FBXO32. Component of the probable SCF(FBXO7) complex containing FBXO7. Component of the SCF(FBXO11) complex containing FBXO11. Component of the SCF(FBXO25) complex containing FBXO25. Component of the SCF(FBXO33) complex containing FBXO33. Component of the probable SCF(FBXO4) complex containing FBXO4. Interacts with RNF7. Part of a complex with TIP120A/CAND1 and RBX1. The unneddylated form interacts with TIP120A/CAND1 and the interaction negatively regulates the association with SKP1 in the SCF complex. Interacts with COPS2. Can self-associate.,tissue specificity:Expressed in lung fibroblasts.,

The CUL-1 Polyclonal Antibody is a highly specific and reliable tool designed for the detection and analysis of CUL-1 protein expression in various biological samples. This antibody is produced using advanced immunization techniques, ensuring high affinity and specificity towards the target protein.

The CUL-1 Polyclonal Antibody exhibits exceptional sensitivity, enabling accurate and precise quantification of CUL-1 protein levels. It has been extensively validated for use in various applications, including Western blotting, immunohistochemistry, and immunofluorescence.

With its superior performance, this antibody allows for the identification and characterization of CUL-1 protein in diverse research areas, such as cancer biology, cell signaling, and protein degradation pathways. Its broad reactivity across multiple species further enhances its versatility and applicability in various experimental settings.

The CUL-1 Polyclonal Antibody is supplied as a liquid formulation, ensuring ease of use and convenience. It is provided in a ready-to-use format, eliminating the need for time-consuming and complex preparation steps. The antibody is also available in different sizes to accommodate varying experimental requirements.

In summary, the CUL-1 Polyclonal Antibody is a highly reliable and specific tool for the detection and analysis of CUL-1 protein expression. Its exceptional sensitivity, broad reactivity, and validated performance make it an indispensable asset for researchers in their pursuit of scientific advancements.

The CUL-1 Polyclonal Antibody is a highly specific and sensitive antibody designed for use in various research applications. This antibody is produced using high-quality polyclonal antibodies that have been purified and tested to ensure optimal performance. The CUL-1 Polyclonal Antibody is designed to detect the CUL-1 protein, which is a key component of the ubiquitin ligase complex. This protein plays a crucial role in the regulation of protein degradation and is involved in various cellular processes, including cell cycle progression, DNA repair, and apoptosis. The CUL-1 Polyclonal Antibody is suitable for use in Western blotting, immunohistochemistry, and immunofluorescence assays. It is supplied in a convenient format and is ready to use, making it an ideal choice for researchers looking to study the function and regulation of the CUL-1 protein. With its high specificity and sensitivity, the CUL-1 Polyclonal Antibody is an essential tool for any researcher working in the field of protein degradation and cellular regulation.