CEP55(Phospho Ser425) Polyclonal Antibody

Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.,PTM:There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully.,subcellular location:Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loss of affinity for the centrosome at the onset of prophase and diffusion throughout the cell. This dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis.,subunit:Homodimer. Interacts (phosphorylated on Ser-425 and Ser-428) with PLK1. Interacts with AKAP9; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PCNT; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PDCD6IP; the interaction is direct; CEP55 binds PDCD6IP in a 2:1 stoechiometry; PDCD6IP competes with TSG101 for the same binding site. Interacts with TSG101; TSG101 competes with PDCD6IP for the same binding site; interaction is required for cytokinesis but not for viral budding. Interacts with FAM125A, VPS37B, VPS37C and VPS28.,tissue specificity:Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain.,

The CEP55(PHOSPHO SER425) Polyclonal Antibody is a highly specific and sensitive tool designed for the detection and analysis of CEP55 protein phosphorylated at serine 425. This antibody is produced using a polyclonal approach, ensuring a diverse range of antibodies that recognize different epitopes on the target protein.

The CEP55 protein plays a crucial role in various cellular processes, including cell division and cytokinesis. Phosphorylation of CEP55 at serine 425 has been implicated in regulating its function and localization during mitosis. Therefore, the CEP55(PHOSPHO SER425) Polyclonal Antibody is an invaluable tool for researchers studying cell division and its associated signaling pathways.

This antibody has been rigorously tested for its specificity and sensitivity in various experimental conditions, including Western blotting, immunoprecipitation, and immunofluorescence. It exhibits minimal cross-reactivity with other proteins, ensuring accurate and reliable results.

The CEP55(PHOSPHO SER425) Polyclonal Antibody is supplied as a liquid formulation, making it easy to handle and use. It is provided in a convenient size, allowing for multiple experiments without the need for frequent reordering.

In summary, the CEP55(PHOSPHO SER425) Polyclonal Antibody is a high-quality research tool that enables the precise detection and analysis of phosphorylated CEP55 protein at serine 425. Its specificity, sensitivity, and versatility make it an essential component for researchers investigating cell division and related cellular processes.