RAD9(Phospho Ser272) Rabbit Polyclonal Antibody

Catalytic activity:Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.|Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double| nick| or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.|PTM:Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.|Belongs to the rad9 family.|subunit:Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex| composed of RAD9A| RAD1 and HUS1. The 9-1-1 complex associates with LIG1| POLB| FEN1| RAD17| HDAC1| RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD9A interacts with BCL2L1| FEN1| PRKCD| RAD9B| HUS1| RAD1| ABL1| RPA1| ATAD5 and RPA2.|

The RAD9(PHOSPHO SER272) Rabbit Polyclonal Antibody is a highly specific and sensitive tool designed for the detection and analysis of RAD9 protein phosphorylated at serine 272. This antibody is produced using high-quality rabbit polyclonal antibodies, ensuring reliable and reproducible results.

RAD9 is a crucial protein involved in the DNA damage response pathway, playing a vital role in maintaining genomic stability. Phosphorylation of RAD9 at serine 272 has been implicated in various cellular processes, including cell cycle regulation and DNA repair. Therefore, the RAD9(PHOSPHO SER272) Rabbit Polyclonal Antibody serves as an invaluable tool for researchers studying these important biological processes.

This antibody has been rigorously tested and validated for its specificity and sensitivity in various applications, including Western blotting, immunoprecipitation, and immunofluorescence. It exhibits minimal cross-reactivity with non-phosphorylated RAD9 protein, ensuring accurate and reliable detection of the phosphorylated form.

The RAD9(PHOSPHO SER272) Rabbit Polyclonal Antibody is supplied in a convenient and ready-to-use format, allowing for easy integration into laboratory workflows. It is provided in a liquid formulation, eliminating the need for reconstitution and ensuring consistent performance.

With its exceptional specificity, sensitivity, and reliability, the RAD9(PHOSPHO SER272) Rabbit Polyclonal Antibody is an indispensable tool for researchers investigating the intricate mechanisms of DNA damage response and related cellular processes.