Histone H4 (Acetyl Lys21) Rabbit Polyclonal Antibody

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin| limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation| DNA repair| DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones| also called histone code| and nucleosome remodeling.|PTM:Acetylation at Lys-6| Lys-9| Lys-13 and Lys-17 occurs in coding regions of the genome but not in heterochromatin.|PTM:Citrullination at Arg-4 by PADI4 impairs methylation.|PTM:Monomethylated| dimethylated or trimethylated at Lys-21. Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.|PTM:Monomethylation at Arg-4 by PRMT1 favors acetylation at Lys-9 and Lys-13.

Demethylation is performed by JMJD6.|PTM:Sumoylated| which is associated with transcriptional repression.|PTM:Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.|Belongs to the histone H4 family.|subunit:The nucleosome is a histone octamer containing two molecules each of H2A| H2B| H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.|

cThe Histone H4 (Acetyl Lys21) Rabbit Polyclonal Antibody is a highly specific and reliable tool for the detection and analysis of acetylated Lysine 21 on Histone H4. This antibody has been meticulously developed and validated to ensure exceptional performance in various applications, including Western blotting, immunohistochemistry, and chromatin immunoprecipitation.

Histone H4 is a crucial component of nucleosomes, which are the basic units of chromatin structure. Acetylation of Lysine 21 on Histone H4 has been implicated in the regulation of gene expression and chromatin remodeling. Therefore, the ability to accurately detect and quantify this modification is of utmost importance in understanding the intricate mechanisms underlying epigenetic regulation.

Our Histone H4 (Acetyl Lys21) Rabbit Polyclonal Antibody exhibits high affinity and specificity towards acetylated Lysine 21 on Histone H4. It has been rigorously tested to ensure minimal cross-reactivity with other histone modifications, ensuring reliable and accurate results. Additionally, this antibody demonstrates excellent sensitivity, allowing for the detection of low abundance acetylated Lysine 21 in complex biological samples.

This antibody is supplied as a purified rabbit polyclonal IgG, which has been affinity-purified using an acetylated peptide corresponding to Lysine 21 of Histone H4. It is provided in a liquid format, ready for immediate use in various experimental procedures. Each batch of antibody undergoes stringent quality control measures to guarantee consistent performance and lot-to-lot reproducibility.

In summary, the Histone H4 (Acetyl Lys21) Rabbit Polyclonal Antibody is an indispensable tool for researchers studying epigenetic modifications and chromatin biology. Its exceptional specificity, sensitivity, and reliability make it an ideal choice for a wide range of applications, enabling accurate and insightful investigations into the role of acetylated Lysine 21 on Histone H4 in gene regulation and chromatin dynamics.