HIF-1 alpha (Acetyl Lys532) Rabbit Polyclonal Antibody

Contains two independent C-terminal transactivation domains| NTAD and CTAD| which function synergistically. Their transcriptional activity is repressed by an intervening inhibitory domain (ID).|

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes| including| erythropoietin| glucose transporters| glycolytic enzymes| vascular endothelial growth factor| and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization| tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-

The HIF-1 alpha (Acetyl Lys532) Rabbit Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of HIF-1 alpha protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the acetylated lysine 532 of human HIF-1 alpha protein. The antibody has been purified using protein A affinity chromatography and is supplied as a liquid in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative.

HIF-1 alpha is a transcription factor that plays a crucial role in the regulation of cellular responses to hypoxia. It is involved in the activation of genes that promote angiogenesis, erythropoiesis, and glycolysis, which are essential for cell survival under low oxygen conditions. The acetylation of lysine 532 in HIF-1 alpha has been shown to enhance its stability and transcriptional activity, making it an important target for research in the field of hypoxia biology.

The HIF-1 alpha (Acetyl Lys532) Rabbit Polyclonal Antibody has been validated for use in various applications, including Western blotting, immunoprecipitation, and immunohistochemistry. It has been shown to specifically recognize acetylated HIF-1 alpha protein in human, mouse, and rat samples. This antibody is an essential tool for researchers studying the role of HIF-1 alpha in hypoxia-related diseases, such as cancer, ischemia, and inflammation.