Shh Polyclonal Antibody

This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube| the anterior-posterior limb axis| and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila| this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly| the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product| restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE)| a d

The Shh Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of Sonic Hedgehog (Shh) protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human Shh protein. The resulting polyclonal antibody is purified using protein A affinity chromatography to ensure high specificity and purity.

The Shh Polyclonal Antibody is suitable for use in various applications, including Western blotting, immunohistochemistry, and immunofluorescence. It has been validated for use in human, mouse, and rat samples, and has been shown to detect Shh protein in a variety of tissues, including brain, lung, liver, and kidney.

This antibody is supplied as a liquid in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative. It is recommended to store the antibody at -20°C for long-term storage and to avoid repeated freeze-thaw cycles.

Overall, the Shh Polyclonal Antibody is a reliable and high-quality tool for the detection of Shh protein in various biological samples. Its high specificity and sensitivity make it an ideal choice for researchers studying the role of Shh in development, disease, and other biological processes.