Phospho-Akt (Y315) Polyclonal Antibody

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in AKT1 have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

The Phospho-Akt (Y315) Polyclonal Antibody is a highly specific and sensitive tool designed for the detection and quantification of phosphorylated Akt at tyrosine 315 residue. This antibody is produced using advanced polyclonal antibody technology, ensuring high affinity and specificity towards the target antigen.

Phosphorylation of Akt at tyrosine 315 plays a crucial role in the regulation of various cellular processes, including cell survival, proliferation, and metabolism. The Phospho-Akt (Y315) Polyclonal Antibody enables researchers to investigate the activation status of Akt and its downstream signaling pathways in a wide range of biological samples.

This antibody has been extensively validated for its performance in various applications, including Western blotting, immunohistochemistry, and immunofluorescence. It exhibits exceptional sensitivity and specificity, allowing for accurate and reliable detection of phosphorylated Akt at tyrosine 315 residue.

The Phospho-Akt (Y315) Polyclonal Antibody is supplied as a ready-to-use solution, eliminating the need for time-consuming and complex antibody preparation procedures. It is compatible with a variety of sample types, including cell lysates, tissue extracts, and purified proteins.

With its superior performance and versatility, the Phospho-Akt (Y315) Polyclonal Antibody is an indispensable tool for researchers studying Akt signaling pathways and its involvement in various physiological and pathological processes.