Phospho-ATF-2 (S112) Polyclonal Antibody

ATF2 encodes a transcription factor (activating transcription factor 2) that is a member of the leucine zipper family of DNA binding proteins. Activating transcription factor 2 has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Activating transcription factor 2 binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Activating transcription factor 2 may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this ATF2.

The Phospho-ATF-2 (S112) Polyclonal Antibody is a highly specific and sensitive tool designed for the detection and quantification of phosphorylated ATF-2 at serine 112 residue. This antibody is produced using advanced polyclonal antibody production techniques, ensuring high affinity and specificity towards the target antigen.

ATF-2, also known as activating transcription factor 2, is a transcription factor that plays a crucial role in cellular responses to various stress stimuli. Phosphorylation of ATF-2 at serine 112 residue is known to regulate its transcriptional activity and is involved in multiple cellular processes, including cell proliferation, differentiation, and apoptosis.

The Phospho-ATF-2 (S112) Polyclonal Antibody has been rigorously validated for its specificity and sensitivity through various experimental approaches, including Western blotting, immunohistochemistry, and immunofluorescence. It exhibits minimal cross-reactivity with non-phosphorylated ATF-2 and other related proteins, ensuring accurate and reliable results.

This antibody is supplied as a liquid formulation, ready to use for various applications in molecular biology and biomedical research. It is compatible with a wide range of sample types, including cell lysates, tissue extracts, and purified proteins. The recommended working dilution ranges from 1:100 to 1:1000, depending on the application and sample type.

With its exceptional performance and reliability, the Phospho-ATF-2 (S112) Polyclonal Antibody is an indispensable tool for researchers studying the intricate signaling pathways involving ATF-2 phosphorylation. Its high specificity and sensitivity enable precise detection and quantification of phosphorylated ATF-2, facilitating a deeper understanding of its functional roles in cellular processes and disease mechanisms.

Note: This product is for research use only and is not intended for diagnostic or therapeutic purposes.