Villin Polyclonal Antibody

VIL1 (villin 1) encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon.

The Villin Polyclonal Antibody is a highly specific and sensitive immunological reagent designed for the detection and quantification of villin protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human villin protein. The resulting polyclonal antibody exhibits high affinity and specificity towards villin protein, making it an ideal tool for various research applications.

The Villin Polyclonal Antibody is suitable for use in a variety of immunoassays, including Western blotting, immunohistochemistry, and ELISA. It has been extensively validated for its ability to detect villin protein in various tissues and cell lines, including intestinal epithelial cells, kidney cells, and lung cells. This antibody has also been shown to be highly sensitive, with a detection limit of 0.1 ng/mL.

In addition to its high specificity and sensitivity, the Villin Polyclonal Antibody is also highly reproducible and reliable. It is supplied as a purified IgG fraction, which has been affinity-purified from rabbit serum using a column containing immobilized villin protein. This ensures that the antibody is free from non-specific binding and other contaminants, and that it exhibits consistent performance from batch to batch.

Overall, the Villin Polyclonal Antibody is an excellent tool for researchers studying villin protein and its role in various biological processes. Its high specificity, sensitivity, and reproducibility make it a valuable reagent for a wide range of applications, from basic research to clinical diagnostics.