VEGF-A Polyclonal Antibody

VEGFA (vascular endothelial growth factor A) is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of VEGFA in mice resulted in abnormal embryonic blood vessel formation. VEGFA is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of VEGFA have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.

The VEGF-A Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of Vascular Endothelial Growth Factor A (VEGF-A) in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human VEGF-A. The resulting polyclonal antibody has been purified using protein A affinity chromatography and is suitable for use in various applications, including Western blotting, immunohistochemistry, and ELISA.

The VEGF-A Polyclonal Antibody has been extensively validated for its specificity and sensitivity in detecting VEGF-A in various biological samples, including human, mouse, and rat tissues. This antibody has been shown to recognize both the recombinant and endogenous forms of VEGF-A, making it an ideal tool for studying the expression and regulation of this important growth factor.

In addition to its high specificity and sensitivity, the VEGF-A Polyclonal Antibody is also highly reproducible, ensuring consistent results across multiple experiments. This antibody is supplied in a convenient liquid format and is ready to use without any additional preparation.

Overall, the VEGF-A Polyclonal Antibody is a reliable and effective tool for the detection and quantification of VEGF-A in various biological samples. Its high specificity, sensitivity, and reproducibility make it an ideal choice for researchers studying the role of VEGF-A in various physiological and pathological processes.