V-ATPase C2 Polyclonal Antibody

ATP6V1C2 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A,three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. ATP6V1C2 encodes alternate transcriptional splice variants, encoding different V1 domain C subunit isoforms.

The V-ATPase C2 Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of V-ATPase C2 protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human V-ATPase C2 protein. The antibody has been purified using protein A affinity chromatography and is supplied as a liquid in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative.

The V-ATPase C2 Polyclonal Antibody has been extensively validated for its specificity and sensitivity in various applications, including Western blotting, immunohistochemistry, and immunofluorescence. It has been shown to specifically recognize V-ATPase C2 protein in human, mouse, and rat tissues and cell lines. The antibody has a high affinity for its target protein, with a reported dilution range of 1:1000 to 1:5000 for Western blotting and 1:100 to 1:500 for immunohistochemistry and immunofluorescence.

This antibody is an essential tool for researchers studying the function and regulation of V-ATPase C2 protein in various biological processes, including lysosomal acidification, bone resorption, and tumor metastasis. Its high specificity and sensitivity make it an ideal choice for detecting low levels of V-ATPase C2 protein in complex biological samples. The V-ATPase C2 Polyclonal Antibody is available in various sizes to meet the needs of different research applications.