Txk Polyclonal Antibody

The Tec family of non-receptor tyrosine kinases is composed of six proteins designated Tec, Emt (also known as Itk or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini. Four alternatively spliced forms of Tec are found to be expressed broadly in cells of hematopoietic lineage and hepatocytes. The Emt gene product associates with CD28 and becomes activated subsequent to CD28 ligation. Btk is necessary for proper B cell development, and mutations in the gene encoding Btk have been associated with families suffering from X-linked agammaglobulinemia, also referred to as Bruton's disease. The Bmx protein shares a high degree of homology with Btk and seems to be expressed at highest levels in the heart. Txk expression is T cell-specific, while expression of the Drosophila Tec homolog, Dsrc28C, is developmentally regulated.

The Txk Polyclonal Antibody is a highly specific and sensitive immunological reagent designed for the detection and quantification of Txk protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human Txk protein. The resulting polyclonal antibody exhibits high affinity and specificity towards Txk protein, making it an ideal tool for various applications in research and diagnostics.

The Txk Polyclonal Antibody is validated for use in Western blotting, immunohistochemistry, and immunofluorescence assays. It recognizes a single band of approximately 70 kDa in Western blot analysis of Txk protein expressed in various cell lines and tissues. In addition, this antibody shows strong staining of Txk protein in formalin-fixed, paraffin-embedded human tissues, indicating its suitability for use in clinical diagnostics.

The Txk Polyclonal Antibody is supplied as a purified IgG fraction, which has been affinity-purified from rabbit serum using protein A chromatography. It is provided in a liquid format, ready for use in various applications. The antibody is stable for long-term storage at -20°C and can be used for multiple experiments without loss of activity.

In summary, the Txk Polyclonal Antibody is a reliable and high-quality reagent for the detection and quantification of Txk protein in various biological samples. Its high specificity and sensitivity make it an ideal tool for research and diagnostic applications.