Smad3 Polyclonal Antibody

Mothers against decapentaplegic homolog 3 encoded by SMAD3 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene ‘mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

The Smad3 Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of Smad3 protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human Smad3 protein. The antibody has been purified using protein A affinity chromatography and is supplied as a liquid in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative.

The Smad3 Polyclonal Antibody has been extensively validated for use in various applications, including Western blotting, immunohistochemistry, and immunofluorescence. It has been shown to specifically recognize Smad3 protein in human, mouse, and rat samples, with no cross-reactivity to other Smad family members. The antibody has also been shown to detect endogenous Smad3 protein in various cell lines and tissues.

This antibody is an essential tool for researchers studying the TGF-β signaling pathway and its role in various biological processes, including cell proliferation, differentiation, and apoptosis. It can be used to investigate the expression and localization of Smad3 protein in various tissues and cell types, as well as to study the regulation of Smad3 activity by post-translational modifications and protein-protein interactions.

In summary, the Smad3 Polyclonal Antibody is a reliable and high-quality reagent that provides specific and sensitive detection of Smad3 protein in various biological samples. Its versatility and broad applicability make it an essential tool for researchers studying the TGF-β signaling pathway and its role in various biological processes.