SENP6 Polyclonal Antibody

Ubiquitin-like molecules (UBLs), such as SUMO1 (UBL1; MIM 601912), are structurally related to ubiquitin (MIM 191339) and can be ligated to target proteins in a similar manner as ubiquitin. However, covalent attachment of UBLs does not result in degradation of the modified proteins. SUMO1 modification is implicated in the targeting of RANGAP1 (MIM 602362) to the nuclear pore complex, as well as in stabilization of I-kappa-B-alpha (NFKBIA; MIM 164008) from degradation by the 26S proteasome. Like ubiquitin, UBLs are synthesized as precursor proteins, with 1 or more amino acids following the C-terminal glycine-glycine residues of the mature UBL protein. Thus, the tail sequences of the UBL precursors need to be removed by UBL-specific proteases, such as SENP6, prior to their conjugation to target proteins (Kim et al. 2000 [PubMed 10799485]). SENPs also display isopeptidase activity for deconjugation of SUMO-conjugated substrates (Lima and Reverter, 2008 [PubMed 18799455]).

The SENP6 Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of SENP6 protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human SENP6 protein. The antibody has been purified using protein A affinity chromatography and is supplied as a liquid in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative.

The SENP6 Polyclonal Antibody has been extensively validated for its specificity and sensitivity in various applications, including Western blotting, immunohistochemistry, and immunofluorescence. It recognizes a single band of approximately 110 kDa in Western blot analysis of human, mouse, and rat cell lysates. In addition, the antibody has been shown to be highly specific for SENP6 protein, as it does not cross-react with other members of the SENP family.

This antibody is an essential tool for researchers studying the role of SENP6 in various biological processes, including DNA repair, cell cycle regulation, and gene expression. It can be used to investigate the expression and localization of SENP6 in different tissues and cell types, as well as to study the effects of various treatments and conditions on SENP6 protein levels.

Overall, the SENP6 Polyclonal Antibody is a reliable and high-quality reagent that can provide valuable insights into the function and regulation of SENP6 protein in various biological systems.