RUNX3 Polyclonal Antibody

RUNX3 (runt related transcription factor 3) encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and RUNX3 is frequently deleted or transcriptionally silenced in cancer. Alternative splicing results in multiple transcript variants.

The RUNX3 Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of RUNX3 protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human RUNX3 protein. The antibody has been purified using protein A affinity chromatography and is supplied as a liquid formulation in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative.

The RUNX3 Polyclonal Antibody has been extensively validated for its specificity and sensitivity in various applications, including Western blotting, immunohistochemistry, and immunofluorescence. It recognizes a single band of approximately 50 kDa in Western blot analysis of human, mouse, and rat cell lysates. In addition, it shows strong staining of RUNX3 protein in formalin-fixed, paraffin-embedded human tissues, including stomach, colon, and lung.

This antibody is an essential tool for researchers studying the role of RUNX3 protein in various biological processes, including cell differentiation, apoptosis, and tumorigenesis. It is also useful for diagnostic applications, such as the detection of RUNX3 protein expression in cancer tissues. The RUNX3 Polyclonal Antibody is available in various sizes and formats to meet the needs of different research applications.