Raf-1 Polyclonal Antibody

RAF1 (Raf-1 proto-oncogene, serine/threonine kinase) is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in RAF1 are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

The RAF-1 Polyclonal Antibody is a highly specific and sensitive immunological reagent designed for the detection and quantification of RAF-1 protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human RAF-1 protein. The resulting polyclonal antibody exhibits high affinity and specificity towards RAF-1 protein, making it an ideal tool for various applications in research and diagnostics.

The RAF-1 Polyclonal Antibody is suitable for use in Western blotting, immunohistochemistry, and immunofluorescence assays. It has been validated for use in human, mouse, and rat samples, and has been shown to detect endogenous RAF-1 protein in various cell lines and tissues. This antibody is supplied as a liquid formulation in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative.

The RAF-1 Polyclonal Antibody is a reliable and versatile tool for the detection and quantification of RAF-1 protein in various biological samples. Its high specificity and sensitivity make it an ideal choice for researchers and clinicians working in the fields of cancer biology, signal transduction, and drug discovery.