Rad54B Polyclonal Antibody

The RAD54 homolog B encoded by RAD54B belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.

The Rad54B Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of Rad54B protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human Rad54B protein. The antibody has been purified using protein A affinity chromatography and is supplied as a liquid in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative.

The Rad54B Polyclonal Antibody has been extensively validated for its specificity and sensitivity in various applications, including Western blotting, immunohistochemistry, and immunofluorescence. It recognizes a single band of approximately 70 kDa in Western blot analysis of human, mouse, and rat cell lysates. In addition, it shows strong staining of Rad54B protein in formalin-fixed, paraffin-embedded human tissues, indicating its suitability for use in immunohistochemistry.

This antibody is an essential tool for researchers studying DNA repair and recombination pathways, as Rad54B is a key player in these processes. Its high specificity and sensitivity make it an ideal choice for detecting Rad54B protein in various biological samples. The Rad54B Polyclonal Antibody is available in various sizes to meet the needs of different research applications.