Rad17 Polyclonal Antibody

The protein encoded by RAD17 (RAD17 checkpoint clamp loader component) is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

The Rad17 Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of Rad17 protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human Rad17 protein. The Rad17 Polyclonal Antibody has been extensively validated for its specificity and sensitivity in Western blotting, immunoprecipitation, and immunofluorescence assays.

It recognizes endogenous Rad17 protein in human, mouse, and rat tissues and cell lines. The Rad17 Polyclonal Antibody is an essential tool for researchers studying DNA damage response and checkpoint signaling pathways. It is supplied as a purified IgG fraction in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative. The Rad17 Polyclonal Antibody is available in various sizes to meet the needs of different applications. With its high quality and reliability, the Rad17 Polyclonal Antibody is an excellent choice for researchers in the field of molecular biology and biotechnology.