PTEN Polyclonal Antibody

PTEN (phosphatase and tensin homolog) was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by PTEN is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of PTEN is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.

The PTEN Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of PTEN protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human PTEN protein. The PTEN Polyclonal Antibody has been extensively validated for its specificity and sensitivity in Western blotting, immunohistochemistry, and immunofluorescence assays.

It recognizes both the full-length and truncated forms of PTEN protein and has been shown to cross-react with PTEN from various species, including human, mouse, and rat. This antibody is an essential tool for researchers studying the role of PTEN in various cellular processes, including cell growth, differentiation, and apoptosis. It is supplied as a purified IgG fraction in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative. The PTEN Polyclonal Antibody is a reliable and high-quality reagent that provides accurate and reproducible results, making it an ideal choice for research applications.