PKA Alpha/Beta cat Polyclonal Antibody

PRKACA encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of PRKACA caused either by somatic mutations, or genomic duplications of regions that include PRKACA, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.

The PKA Alpha/Beta cat Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of protein kinase A (PKA) alpha and beta catalytic subunits in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of PKA alpha and beta catalytic subunits. The resulting polyclonal antibody exhibits high affinity and specificity towards PKA alpha and beta catalytic subunits, making it an ideal tool for various applications such as western blotting, immunoprecipitation, and immunohistochemistry. The PKA Alpha/Beta cat Polyclonal Antibody is supplied as a liquid formulation in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative. This antibody is suitable for use in both research and diagnostic applications and is available in various sizes to meet the needs of different experimental setups. With its high specificity and sensitivity, the PKA Alpha/Beta cat Polyclonal Antibody is an essential tool for researchers studying PKA signaling pathways and related biological processes.