PCAF Polyclonal Antibody

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.

The PCAF Polyclonal Antibody is a highly specific and sensitive immunological reagent designed for the detection and quantification of PCAF protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human PCAF protein, followed by affinity purification using a column containing the same peptide. The resulting antibody exhibits high affinity and specificity towards PCAF protein, with minimal cross-reactivity to other related proteins.

The PCAF Polyclonal Antibody is suitable for a wide range of applications, including Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry. It has been validated for use in human, mouse, and rat samples, and has been shown to detect endogenous levels of PCAF protein in various cell lines and tissues.

This antibody is supplied as a liquid solution in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative. It is available in various sizes and formats to meet the needs of different experimental setups. The PCAF Polyclonal Antibody is a reliable and effective tool for studying the function and regulation of PCAF protein in various biological processes, including gene transcription, DNA repair, and cell differentiation.