p130 Polyclonal Antibody

The retinoblastoma (Rb) tumor suppressor family includes the retinoblastoma protein Rb (p105), retinoblastoma-like protein 1 (RBL1, p107), and retinoblastoma-like protein 2 (RBL2, p130). These Rb family proteins are referred to as ‘pocket proteins’ because they contain a conserved binding pocket region that interacts with critical regulatory proteins, including E2F family transcription factors, c-Abl tyrosine kinase, and proteins containing a conserved LXCXE motif. In quiescent G0 phase cells, active Rb proteins are hypophosphorylated and bind to E2F transcription factors to repress transcription and inhibit cell cycle progression. Upon growth factor induction of quiescent cells, Rb proteins become hyperphosphorylated and inactivated by G1-phase cyclinD-cdk4/6, G1/S-phase cyclin E-cdk2, and G1/S-phase cyclin A-cdk2 complexes. Hyperphosphorylation of Rb proteins results in a loss of E2F binding and allows for transcriptional activation and cell cycle progression. In addition to regulating the cell cycle, Rb proteins regulate chromosome stability, induction, and maintenance of senescence, apoptosis, cellular differentiation, and angiogenesis. Retinoblastoma-like protein 2 (RBL2, p130) is the most predominant and active Rb family member found in quiescent cells. In these cells, RBL2 interacts with E2F4 and E2F5 to recruit the DP, RB-like, E2F, and MuvB protein (DREAM) complex to E2F target genes to repress transcription of multiple genes required for progression into S phase and mitosis. Hypophosphorylation of RBL2 during cellular senescence is required for maintenance of senescent cells.

The P130 Polyclonal Antibody is a highly specialized and meticulously crafted product designed to cater to the needs of researchers and scientists in the field of biotechnology. This antibody is derived from a diverse pool of immunoglobulins, ensuring a broad spectrum of reactivity and enhanced sensitivity in various applications.

With its exceptional specificity and affinity, the P130 Polyclonal Antibody offers unparalleled performance in immunohistochemistry, immunofluorescence, and Western blotting techniques. Its robust nature allows for reliable and reproducible results, making it an indispensable tool for accurate and precise experimental outcomes.

The P130 Polyclonal Antibody is meticulously manufactured using state-of-the-art techniques, ensuring the highest level of purity and quality. Rigorous quality control measures are implemented throughout the production process to guarantee batch-to-batch consistency and reliability.

This antibody is supplied in a convenient and user-friendly format, allowing for easy handling and storage. Its long shelf life ensures prolonged usability, making it a cost-effective choice for laboratories and research institutions.

In summary, the P130 Polyclonal Antibody is a cutting-edge product that offers exceptional performance, reliability, and versatility. Its broad reactivity and high sensitivity make it an invaluable asset for researchers seeking accurate and reproducible results in their biotechnological endeavors.