Nup93 Polyclonal Antibody

The nuclear pore complex (NPC) mediates bidirectional macromolecular traffic between the nucleus and cytoplasm in eukaryotic cells and is comprised of more than 100 different subunits. Many of the subunits belong to a family called nucleoporins (Nups), which are characterized by the presence of O-linked-N-acetylglucosamine moieties and a distinctive pentapeptide repeat (XFXFG). Nup93 (nucleoporin 93) is the most abundant nucleoporin found per NPC, contributing over 10% of the mass. It localizes to the nuclear side of the NPC, predominantly in the basket terminal ring area, and exists in a complex with Nup188, Nup53 and Nup205. This complex is crucial for NPC stability and proper assembly. Nup93 interacts directly with the Nup62 complex located at the center of the NPC and thus tethers the two subcomplexes. Nup93 is composed of a coiled-coil domain at its N-terminus and a C-terminal helical domain. Its proper function is essential for cell viability and normal NPC function.

The Nup93 Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of Nup93 protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the N-terminal region of human Nup93 protein. The antibody has been purified using protein A affinity chromatography and is supplied as a liquid in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative.

The Nup93 Polyclonal Antibody has been extensively validated for use in various applications, including Western blotting, immunohistochemistry, and immunofluorescence. It has been shown to specifically recognize Nup93 protein in various cell lines and tissues, including human, mouse, and rat. The antibody has also been shown to be highly sensitive, with a detection limit of 0.1 ng of Nup93 protein.

This antibody is an essential tool for researchers studying the nuclear pore complex and its role in nucleocytoplasmic transport. It can be used to investigate the expression and localization of Nup93 protein in various cell types and tissues, as well as to study the function of Nup93 in nuclear transport and other cellular processes. The Nup93 Polyclonal Antibody is a reliable and high-quality reagent that will enable researchers to advance their understanding of the molecular mechanisms underlying cellular processes.