Histone H4 (Acetyl Lys5) Polyclonal Antibody

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST4H4 is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from HIST4H4 lack polyA tails; instead, they contain a palindromic termination element.

The Histone H4 (Acetyl Lys5) Polyclonal Antibody is a highly specific and sensitive tool designed for the detection and analysis of acetylated lysine 5 on histone H4. This antibody is produced using advanced techniques and rigorous quality control measures to ensure optimal performance and reliability.

Histone H4 is a core component of nucleosomes, which are the basic units of chromatin structure. Acetylation of lysine residues on histone proteins plays a crucial role in regulating gene expression and chromatin remodeling. The acetylation of lysine 5 on histone H4 has been implicated in various biological processes, including transcriptional activation and DNA repair.

This polyclonal antibody is raised in rabbits using a synthetic peptide corresponding to the acetylated lysine 5 region of histone H4. It has been extensively validated for specificity and sensitivity through various experimental approaches, including Western blotting, immunohistochemistry, and immunofluorescence.

The Histone H4 (Acetyl Lys5) Polyclonal Antibody offers several advantages over other commercially available antibodies. It exhibits high affinity and specificity towards acetylated lysine 5 on histone H4, ensuring accurate and reliable detection. It also demonstrates minimal cross-reactivity with other acetylated lysine residues or non-acetylated forms of histone H4.

This antibody is supplied as a liquid formulation in a convenient size, allowing for easy handling and storage. It is provided in a ready-to-use format, eliminating the need for time-consuming and complex preparation steps. The antibody is suitable for use in various applications, including Western blotting, immunohistochemistry, and immunofluorescence, making it a versatile tool for researchers studying epigenetics, chromatin biology, and gene regulation.

In summary, the Histone H4 (Acetyl Lys5) Polyclonal Antibody is a highly reliable and specific reagent for the detection and analysis of acetylated lysine 5 on histone H4. Its superior performance, ease of use, and broad applicability make it an essential tool for researchers in the field of molecular biology and epigenetics.