Histone H3 Polyclonal Antibody

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

The Histone H3 Polyclonal Antibody is a highly specific and sensitive antibody that is designed to detect and quantify histone H3 protein in various biological samples. This antibody is produced using high-quality polyclonal antibodies that are generated by immunizing rabbits with a synthetic peptide corresponding to the N-terminal region of human histone H3 protein. The antibody is purified using a combination of protein A and protein G chromatography to ensure high purity and specificity.

The Histone H3 Polyclonal Antibody is suitable for use in a wide range of applications, including western blotting, immunohistochemistry, and immunofluorescence. It has been extensively validated for use in various species, including human, mouse, and rat, and has been shown to exhibit excellent sensitivity and specificity in detecting histone H3 protein.

This antibody is supplied as a liquid solution in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative. It is available in various sizes to meet the needs of different research applications. The Histone H3 Polyclonal Antibody is an essential tool for researchers studying epigenetics, chromatin biology, and gene regulation. Its high specificity and sensitivity make it an ideal choice for detecting and quantifying histone H3 protein in various biological samples.