Histone H3 (Di Methyl Lys5) Polyclonal Antibody

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST1H3A is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from HIST1H3A lack polyA tails; instead, they contain a palindromic termination element. HIST1H3A is found in the large histone gene cluster on chromosome 6p22-p21.3.

The Histone H3 (Di Methyl Lys5) Polyclonal Antibody is a highly specific and sensitive tool for the detection and analysis of histone H3 protein modifications. This antibody is designed to specifically recognize and bind to the di-methylated lysine 5 residue on histone H3, allowing for the precise identification and quantification of this specific modification.

Histone proteins play a crucial role in the regulation of gene expression and chromatin structure. Post-translational modifications of histones, such as methylation, acetylation, and phosphorylation, have been shown to have a significant impact on gene expression and cellular processes. The di-methylation of lysine 5 on histone H3 is known to be involved in various biological processes, including transcriptional activation and repression, DNA repair, and cell cycle regulation.

The Histone H3 (Di Methyl Lys5) Polyclonal Antibody has been extensively validated for its specificity and sensitivity in various applications, including Western blotting, immunohistochemistry, and chromatin immunoprecipitation (ChIP). It exhibits minimal cross-reactivity with other histone modifications, ensuring accurate and reliable results.

This antibody is produced using high-quality immunogens and state-of-the-art purification techniques, ensuring high affinity and specificity. It is supplied as a ready-to-use solution, allowing for convenient and efficient use in various experimental settings.

In summary, the Histone H3 (Di Methyl Lys5) Polyclonal Antibody is a valuable tool for researchers studying histone modifications and their role in gene regulation and cellular processes. Its high specificity, sensitivity, and ease of use make it an ideal choice for a wide range of applications.