CYP17A1 Polyclonal Antibody

CYP17A1 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia.

The CYP17A1 Polyclonal Antibody is a highly specific and reliable tool designed for the detection and quantification of CYP17A1 protein in various biological samples. This antibody has been extensively validated for its exceptional performance in immunohistochemistry (IHC), immunofluorescence (IF), and western blotting (WB) applications.

CYP17A1, also known as cytochrome P450 17A1, plays a crucial role in steroidogenesis by catalyzing two key reactions in the biosynthesis of steroid hormones. It acts as both a 17α-hydroxylase and a 17,20-lyase, converting pregnenolone and progesterone into their respective downstream products, ultimately leading to the production of androgens and estrogens.

The CYP17A1 Polyclonal Antibody has been meticulously developed using high-quality immunogens and advanced immunization techniques. It exhibits exceptional specificity towards CYP17A1, ensuring minimal cross-reactivity with other related proteins. This antibody demonstrates high sensitivity, enabling the detection of even low levels of CYP17A1 protein in complex biological samples.

In IHC applications, the CYP17A1 Polyclonal Antibody provides clear and reliable staining results, allowing for the precise localization of CYP17A1 protein within tissues. It is suitable for use in various tissue types, including formalin-fixed paraffin-embedded (FFPE) sections. Additionally, this antibody is compatible with multiple detection systems, facilitating flexibility in experimental design.

For IF applications, the CYP17A1 Polyclonal Antibody exhibits strong and specific fluorescence signals, enabling the visualization and analysis of CYP17A1 protein in cells and tissues. It is compatible with a wide range of fluorophores, allowing for multiplexing experiments and co-localization studies.

In WB applications, the CYP17A1 Polyclonal Antibody reliably detects the target protein at the expected molecular weight, providing accurate quantification of CYP17A1 expression levels. It exhibits excellent signal-to-noise ratio and minimal background interference, ensuring reliable and reproducible results.

Overall, the CYP17A1 Polyclonal Antibody is a valuable tool for researchers studying steroidogenesis, endocrinology, and related fields. Its exceptional specificity, sensitivity, and versatility make it an ideal choice for various applications, including IHC, IF, and WB.