Cyclin D1 Polyclonal Antibody

Cyclin D1 encoded by CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. Cyclin D1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of CCND1, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.

The Cyclin D1 Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of Cyclin D1 protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the N-terminal region of human Cyclin D1 protein. The antibody has been purified using protein A affinity chromatography and is supplied as a liquid formulation in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative.

The Cyclin D1 Polyclonal Antibody is suitable for use in various applications, including Western blotting, immunohistochemistry, and immunofluorescence. It has been validated for use in human, mouse, and rat samples and has been shown to exhibit high specificity and sensitivity in detecting Cyclin D1 protein.

This antibody is an essential tool for researchers studying cell cycle regulation, cancer biology, and other related fields. Its high specificity and sensitivity make it an ideal choice for detecting Cyclin D1 protein in various biological samples, including tissue lysates, cell lysates, and formalin-fixed paraffin-embedded (FFPE) tissue sections.

In summary, the Cyclin D1 Polyclonal Antibody is a reliable and effective tool for the detection of Cyclin D1 protein in various biological samples. Its high specificity and sensitivity make it an essential tool for researchers studying cell cycle regulation and cancer biology.