COX7a2/3 Polyclonal Antibody

Cytochrome c oxidase, the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of three catalytic subunits encoded by mitochondrial genes, and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, while the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (liver isoform) of subunit VIIa, with this polypeptide being present in both muscle and non-muscle tissues. In addition to polypeptide 2, subunit VIIa includes polypeptide 1 (muscle isoform), which is present only in muscle tissues, and a related protein, which is present in all tissues. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 4 and 14.

The COX7a2/3 Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of COX7a2/3 protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human COX7a2/3 protein. The antibody has been purified using protein A affinity chromatography and is supplied as a liquid in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative.

The COX7a2/3 Polyclonal Antibody has been extensively validated for use in various applications, including Western blotting, immunohistochemistry, and immunofluorescence. It has been shown to specifically recognize COX7a2/3 protein in human, mouse, and rat tissues, with minimal cross-reactivity to other proteins. The antibody has also been shown to be highly sensitive, with a detection limit of 0.1 ng of COX7a2/3 protein.

This antibody is an essential tool for researchers studying mitochondrial function and metabolism, as COX7a2/3 is a critical subunit of cytochrome c oxidase, the terminal enzyme of the mitochondrial respiratory chain. The COX7a2/3 Polyclonal Antibody can be used to investigate the expression and localization of COX7a2/3 protein in various tissues and cell types, as well as to study the role of COX7a2/3 in mitochondrial function and disease.

In summary, the COX7a2/3 Polyclonal Antibody is a highly specific and sensitive antibody that is ideal for the detection of COX7a2/3 protein in various biological samples. Its extensive validation and broad range of applications make it an essential tool for researchers studying mitochondrial function and metabolism.