COX5a Polyclonal Antibody

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. COX5A (cytochrome c oxidase subunit 5A) encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22.

The COX5a Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of COX5a protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human COX5a protein. The antibody has been purified using protein A affinity chromatography and is supplied as a liquid in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative.

The COX5a Polyclonal Antibody has been extensively validated for use in various applications, including Western blotting, immunohistochemistry, and immunofluorescence. It has been shown to specifically recognize COX5a protein in human, mouse, and rat tissues, making it a versatile tool for researchers studying mitochondrial function and metabolism.

This antibody is highly sensitive, with a detection limit of 0.1 ng for Western blotting and 1 ng for immunohistochemistry. It has also been shown to have low cross-reactivity with other proteins, ensuring accurate and reliable results.

Overall, the COX5a Polyclonal Antibody is a high-quality research tool that can be used to study the role of COX5a protein in various biological processes. Its high specificity, sensitivity, and low cross-reactivity make it an ideal choice for researchers looking to accurately detect and quantify COX5a protein in their samples.