Cleaved-Factor Xa activated HC (I235) Polyclonal Antibody

F10 encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.

The Cleaved-Factor Xa activated HC (I235) Polyclonal Antibody is a highly specific and sensitive immunological reagent designed for the detection and quantification of activated HC (I235) in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the cleaved form of Factor Xa activated HC (I235) and purified using a combination of protein A and peptide affinity chromatography techniques.

The Cleaved-Factor Xa activated HC (I235) Polyclonal Antibody exhibits excellent specificity and sensitivity towards its target antigen, with minimal cross-reactivity towards other related proteins. It is suitable for use in a wide range of applications, including Western blotting, ELISA, immunohistochemistry, and flow cytometry.

This antibody is supplied as a liquid formulation in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative. It is recommended to store the antibody at -20°C for long-term storage and avoid repeated freeze-thaw cycles.

In summary, the Cleaved-Factor Xa activated HC (I235) Polyclonal Antibody is a reliable and high-quality reagent that can facilitate the accurate detection and quantification of activated HC (I235) in various biological samples. Its excellent specificity and sensitivity make it an ideal tool for researchers and clinicians working in the fields of coagulation, thrombosis, and hemostasis.