Cleaved-Caspase-7 (S199) Polyclonal Antibody

CASP7 encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded caspase 7 is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for CASP7.

The Cleaved-Caspase-7 (S199) Polyclonal Antibody is a highly specific and sensitive tool designed for the detection and quantification of cleaved caspase-7 (S199) in various biological samples. This antibody is produced using advanced immunization techniques, ensuring high affinity and specificity towards the target protein.

Caspase-7 is a crucial enzyme involved in the execution phase of apoptosis, playing a pivotal role in cellular homeostasis and tissue development. The cleavage of caspase-7 at serine 199 is a key event in its activation, leading to the initiation of apoptotic processes. Therefore, the detection and quantification of cleaved caspase-7 (S199) is of great importance in understanding the mechanisms underlying apoptosis and its dysregulation in various diseases.

The Cleaved-Caspase-7 (S199) Polyclonal Antibody exhibits exceptional sensitivity and specificity, enabling accurate and reliable detection of cleaved caspase-7 (S199) in a wide range of sample types, including cell lysates, tissue homogenates, and biological fluids. This antibody has been extensively validated through various experimental approaches, ensuring its reliability and reproducibility.

Furthermore, the Cleaved-Caspase-7 (S199) Polyclonal Antibody demonstrates excellent performance in immunohistochemistry (IHC), immunofluorescence (IF), and western blotting applications. It exhibits minimal cross-reactivity with other caspase family members, ensuring specific detection of cleaved caspase-7 (S199) without interference from related proteins.

The Cleaved-Caspase-7 (S199) Polyclonal Antibody is supplied as a ready-to-use solution, eliminating the need for time-consuming and error-prone antibody preparation. It is provided in a convenient format, allowing for easy and efficient incorporation into various experimental protocols.

In summary, the Cleaved-Caspase-7 (S199) Polyclonal Antibody is a highly reliable and specific tool for the detection and quantification of cleaved caspase-7 (S199). Its exceptional sensitivity, specificity, and versatility make it an invaluable asset for researchers studying apoptosis and its implications in various pathological conditions.